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dnazol direct dn131  (Molecular Research Center inc)

 
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    Molecular Research Center inc dnazol direct dn131
    Dnazol Direct Dn131, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dn131/dnazol+direct/pm37552984-277-23-17
    Average 90 stars, based on 1 article reviews
    dnazol direct dn131 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards
    Article Snippet: paper cards provide an effective DNA storage method. .. In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago. .. These DNA samples were used as templates for amplification

    Amplification:

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards
    Article Snippet: paper cards provide an effective DNA storage method. .. In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago. .. These DNA samples were used as templates for amplification

    Agarose Gel Electrophoresis:

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards
    Article Snippet: paper cards provide an effective DNA storage method. .. In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago. .. These DNA samples were used as templates for amplification

    Staining:

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards
    Article Snippet: paper cards provide an effective DNA storage method. .. In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago. .. These DNA samples were used as templates for amplification

    Genotyping Assay:

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards
    Article Snippet: paper cards provide an effective DNA storage method. .. In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago. .. These DNA samples were used as templates for amplification

    DNA Extraction:

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards
    Article Snippet: paper cards provide an effective DNA storage method. .. In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago. .. These DNA samples were used as templates for amplification



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    dn131  (Molecular Research Center inc)
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    Concentrations, A260/A280 and A260/A230 ratios of DNA isolated from old human blood paper cards with the four different reagents <t>(DN131,</t> Tris–HCl, TE, H 2 O). A . DNA concentrations (ng/μl); B . A260/A280 ratios; C . A260/A230 ratios. *, p < 0.05, compared to DN131 group. T -test was performed for DNA concentrations and A260/A230 ratios because these data are normally distributed, while A260/A280 data are not normally distributed. Wilcoxon Rank-Sum test was used to examine the A260/A280 differences between methods including DN131.
    Dn131, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Concentrations, A260/A280 and A260/A230 ratios of DNA isolated from old human blood paper cards with the four different reagents (DN131, Tris–HCl, TE, H 2 O). A . DNA concentrations (ng/μl); B . A260/A280 ratios; C . A260/A230 ratios. *, p < 0.05, compared to DN131 group. T -test was performed for DNA concentrations and A260/A230 ratios because these data are normally distributed, while A260/A280 data are not normally distributed. Wilcoxon Rank-Sum test was used to examine the A260/A280 differences between methods including DN131.

    Journal: SpringerPlus

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards

    doi: 10.1186/2193-1801-2-616

    Figure Lengend Snippet: Concentrations, A260/A280 and A260/A230 ratios of DNA isolated from old human blood paper cards with the four different reagents (DN131, Tris–HCl, TE, H 2 O). A . DNA concentrations (ng/μl); B . A260/A280 ratios; C . A260/A230 ratios. *, p < 0.05, compared to DN131 group. T -test was performed for DNA concentrations and A260/A230 ratios because these data are normally distributed, while A260/A280 data are not normally distributed. Wilcoxon Rank-Sum test was used to examine the A260/A280 differences between methods including DN131.

    Article Snippet: In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago.

    Techniques: Isolation

    Bland-Altman Plots of DNA concentrations, A260/A280 and A260/A230 ratios between the two methods using DN131 and using our solutions (Tris–HCl, TE or water). A . Bland-Altman Plot of DNA concentrations between DN131 and Tris–HCl. Correlation R = 0.38 ( p < 0.0001), slope = − 0.76 ( p < 0.001), intercept = 193.27 ( p < 0.0001); B . Bland-Altman Plot of DNA concentrations between DN131 and TE. Correlation R = 0.28 ( p < 0.004), slope = − 0.46 ( p = 0.004), intercept = 145.59 ( p < 0.0001); C . Bland-Altman Plot of DNA concentrations between DN131 and H 2 O. Correlation R = 0.05 ( p = 0.55), slope = 0.32 ( p = 0.96), intercept = 59.65 ( p = 0.99); D . Bland-Altman Plot of A260/A280 ratios between DN131 and Tris–HCl. Correlation R = 0.99 ( p < 0.0001), slope = − 2.0 ( p < 0.0001), intercept = 2.95 ( p < 0.0001); E . Bland-Altman Plot of A260/A280 ratios between DN131 and TE. Correlation R = 0.97 ( p < 0.0001), slope = − 1.97 ( p < 0.0001), intercept = 3.51 ( p < 0.0001); F . Bland-Altman Plot of A260/A280 ratios between DN131 and H 2 O. Correlation R = 0.99 ( p < 0.0001), slope = −20 ( p < 0.0001), intercept = 2.91 ( p < 0.0001); G . Bland-Altman Plot of A260/A230 ratios between DN131 and Tris–HCl. Correlation R = 0.06 ( p = 0.57), slope = 0.64 ( p = 0.99), intercept = 0.27 ( p = 0.99); H . Bland-Altman Plot of A260/A230 ratios between DN131 and TE. Correlation R = 0.99 ( p < 0.0001), slope = 1.99 ( p < 0.0001), intercept = −0.12 ( p = 0.007); I . Bland-Altman Plot of A260/A230 ratios between DN131 and H 2 O. Correlation R = 0.47 ( p < 0.0001), slope = 0.97 ( p < 0.0001), intercept = 0.13 ( p = 0.007).

    Journal: SpringerPlus

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards

    doi: 10.1186/2193-1801-2-616

    Figure Lengend Snippet: Bland-Altman Plots of DNA concentrations, A260/A280 and A260/A230 ratios between the two methods using DN131 and using our solutions (Tris–HCl, TE or water). A . Bland-Altman Plot of DNA concentrations between DN131 and Tris–HCl. Correlation R = 0.38 ( p < 0.0001), slope = − 0.76 ( p < 0.001), intercept = 193.27 ( p < 0.0001); B . Bland-Altman Plot of DNA concentrations between DN131 and TE. Correlation R = 0.28 ( p < 0.004), slope = − 0.46 ( p = 0.004), intercept = 145.59 ( p < 0.0001); C . Bland-Altman Plot of DNA concentrations between DN131 and H 2 O. Correlation R = 0.05 ( p = 0.55), slope = 0.32 ( p = 0.96), intercept = 59.65 ( p = 0.99); D . Bland-Altman Plot of A260/A280 ratios between DN131 and Tris–HCl. Correlation R = 0.99 ( p < 0.0001), slope = − 2.0 ( p < 0.0001), intercept = 2.95 ( p < 0.0001); E . Bland-Altman Plot of A260/A280 ratios between DN131 and TE. Correlation R = 0.97 ( p < 0.0001), slope = − 1.97 ( p < 0.0001), intercept = 3.51 ( p < 0.0001); F . Bland-Altman Plot of A260/A280 ratios between DN131 and H 2 O. Correlation R = 0.99 ( p < 0.0001), slope = −20 ( p < 0.0001), intercept = 2.91 ( p < 0.0001); G . Bland-Altman Plot of A260/A230 ratios between DN131 and Tris–HCl. Correlation R = 0.06 ( p = 0.57), slope = 0.64 ( p = 0.99), intercept = 0.27 ( p = 0.99); H . Bland-Altman Plot of A260/A230 ratios between DN131 and TE. Correlation R = 0.99 ( p < 0.0001), slope = 1.99 ( p < 0.0001), intercept = −0.12 ( p = 0.007); I . Bland-Altman Plot of A260/A230 ratios between DN131 and H 2 O. Correlation R = 0.47 ( p < 0.0001), slope = 0.97 ( p < 0.0001), intercept = 0.13 ( p = 0.007).

    Article Snippet: In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago.

    Techniques:

    PCR amplification of a DNA fragment of caspase-12 gene covering C125T SNP (rs497116) using 105 DNA samples isolated by different reagents (DN131, Tris–HCl, TE and water) with two different PCR amplification systems. A . A representative image of PCR amplified bands separated by a 2% agarose gel electrophoresis for system 1, which was detailed in Materials and methods. PCR bands were visualized under UV light after ethidium bromide staining; B . PCR band density quantification by Image J for Figure 3A. *, p < 0.05; **, p < 0.01, compared to DN131; C . A representative image of PCR amplified bands separated by a 2% agarose gel electrophoresis for system 2, which was detailed in Materials and methods; D . PCR band density quantification by Image J for Figure 3C. ***, p < 0.001, compared to DN131.

    Journal: SpringerPlus

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards

    doi: 10.1186/2193-1801-2-616

    Figure Lengend Snippet: PCR amplification of a DNA fragment of caspase-12 gene covering C125T SNP (rs497116) using 105 DNA samples isolated by different reagents (DN131, Tris–HCl, TE and water) with two different PCR amplification systems. A . A representative image of PCR amplified bands separated by a 2% agarose gel electrophoresis for system 1, which was detailed in Materials and methods. PCR bands were visualized under UV light after ethidium bromide staining; B . PCR band density quantification by Image J for Figure 3A. *, p < 0.05; **, p < 0.01, compared to DN131; C . A representative image of PCR amplified bands separated by a 2% agarose gel electrophoresis for system 2, which was detailed in Materials and methods; D . PCR band density quantification by Image J for Figure 3C. ***, p < 0.001, compared to DN131.

    Article Snippet: In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago.

    Techniques: Amplification, Isolation, Agarose Gel Electrophoresis, Staining

    Success rates of amplifying caspase-12 C125T SNP (rs497116) by PCR and a Taqman genotyping assay using four different reagents

    Journal: SpringerPlus

    Article Title: A reliable and effective method of DNA isolation from old human blood paper cards

    doi: 10.1186/2193-1801-2-616

    Figure Lengend Snippet: Success rates of amplifying caspase-12 C125T SNP (rs497116) by PCR and a Taqman genotyping assay using four different reagents

    Article Snippet: In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris–HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago.

    Techniques: Genotyping Assay